Single Cell Dataset

ControlControl groups tumor were processed into single-cell suspensions when the tumor size of the control group reached 100 mm2. After antibody staining , tumor CD45.2+ cells were sorted on a FACS AriaIII.Ten thousand cells were mixed with 10× Genomics Chromium single-cell RNA mastermix, followed by loading onto a 10× Chromium chip according to the manufacturer’s protocol to obtain single-cell cDNA. Libraries were subsequently prepared and sequenced using a NovaSeq sequencer (Illumina).<br>MK-801Hepa1-6BL (5 × 105) tumor cells were injected subcutaneously (s.c.) into C57BL/6 WT, respectively.Tumor-bearing mice were treated with MK-801 (0.2 mg/kg, i.p. daily from day 5). When the tumor size of the control group reached 100 mm2. After antibody staining , tumor CD45.2+ cells were sorted on a FACS AriaIII. Ten thousand cells were mixed with 10× Genomics Chromium single-cell RNA mastermix, followed by loading onto a 10× Chromium chip according to the manufacturer’s protocol to obtain single-cell cDNA. Libraries were subsequently prepared and sequenced using a NovaSeq sequencer (Illumina).<br>all gene expression.xls （RNA-Seq mouse bone marrow）The bone marrow cells were cultured in complete RPMI 1640 media supplemented with 5% L929 cells supernatant, and 0.1% β-mercaptoethanol for 5 days to generate BMDMs.BMDMs were harvested after 6 h stimulation with MK-801 in the presence of tumor conditioned medium, and total RNA was extracted from the treated cells using TRizol.Total RNA was extracted using Trizol reagent following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit, high-quality RNA samples with RIN number &gt; 7.0 were used to construct sequencing library.