Transcription profiling of wild type yeast grown with ethanol, glucose and galactose and the deletion mutant of Rrp6 to identify transcription start and end positions

To obtain a comprehensive survey of the structure and expression level of transcripts across the yeast genome, we used tiling arrays to profile wild-type transcriptomes in ethanol (YPE), glucose (YPD, SDC), and galactose (YPGal), which together encompass the main laboratory growth conditions of yeast为全面解析酵母全基因组中转录本的结构与表达水平，本研究采用平铺式基因芯片（tiling array），对乙醇（YPE）、葡萄糖（YPD、SDC）及半乳糖（YPGal）三种培养条件下的酵母野生型转录组进行了谱分析。上述三种培养条件覆盖了酵母主要的实验室生长环境。