RNAseq to profile transcriptomes in bone marrow-derived macrophages from Nelfb KO(Nelfbfl/fl, Lyz2-Cre) and WT(Nelfb+/+,Lyz2-Cre) mice

We performed the transcriptomic analysis of RNA-seq of BMDMs generated from WT and Nelfb KO in conditions of unstimuated and LPS stimualtion to identify gene expression changes. BMDMs from WT and KO were left untreated or stimulated with LPS for indicated time. RNA was extracted, polyA enriched and prepared for sequencing libraries.