Kinetic parameters for purified recombinant oxidoreductases with Cr(VI) as substrate.

aEnzyme nomenclature is described in full in Materials and Methods. With the exception of NemA_Ec, all enzyme kinetics were measured with NADH as cofactor on the basis that this cofactor is more cost-effective for high level use than NADPH. NemA_Ec kinetic parameters were measured with both cofactors to enable any preference to be identified.
bApparent KM and kcat as measured at 1 mM NADH (or 1 mM NADPH, for NemA_Ec only). Data are the mean of two independent replicates, and error bars indicate ±1 standard deviation.
cNo error bars are available, as derivation of meaningful values from a Lineweaver-Burk plot for these low activity enzymes required the replicate data to be averaged prior to analysis.