Effect of melamine feeding on the protein level of Juno in ovulated eggs.

(A) Juno was stained with rat monoclonal anti-mouse folr4 antibody and examined by confocal microscopy. Scale bar, 20μm. (B) Measurement of fluorescent intensity of Juno signals. The protein level of Juno in egg membrane was significantly reduced in high-dose group (n = 15) compared to control group (n = 25). Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a P < 0.05 level of significance.