Gene expression profile at single-cell level of regenerating lizard (Anolis carolinensis) tail

Lizards cannot naturally regenerate limbs but are the closest known relatives of mammals capable of epimorphic tail regrowth. However, the mechanisms regulating lizard blastema derivation and chondrogenesis remain unclear. We utilized single-cell RNA sequencing analyses of regenerating lizard tails throughout the course of regeneration to assess diversity and heterogeneity in regeneating tail cell populations. Regenerating green anole lizard (Anolis carolinensis) tail samples were collected 0-28 days post amputation (DPA) and digested with physical and enzymatic dissociation to obtain single-cell suspensions for single-cell RNA sequencing analysis.