Single cell RNA-Seq of the murine non-myocyte cardiac cellulome

We characterized single-cell transcriptional profiles of the cardiac non-myocyte cell pool in C57BL/6J mice. The cell preparation we sequenced consisted of metabolically active, nucleated non-myocyte cells from heart ventricles of female and male mice which were depleted of endothelial cells. The goals of this experiment included examining cellular diversity, identifying markers of understudied cell populations, exploring functional roles of different cell types, and characterizing sexual dimorphism in cardiac gene expression.本研究对C57BL/6J小鼠心脏非心肌细胞群的单细胞转录谱进行了表征。本次测序所用的细胞制备物源自去除了内皮细胞的雌雄小鼠心室，包含代谢活跃的有核非心肌细胞。本实验的研究目标包括：解析细胞多样性、鉴定尚未被充分研究的细胞群体的标志物、探究不同细胞类型的功能作用，以及表征心脏基因表达的性别二态性。