Dynamic regulation of miRNA accumulation during C. elegans larval development [smallRNA-Seq 3]

Here, we profile miRNA expression at high temporal resolution (1 hr interval) during C. elegans postembryonic development, which identifies several miRNAs with highly dynamic concentration changes Overall design: Synchronized L1's of HW1993 (xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV) and HW2706 (lin-28(xe192) I; xeSi312 [Peft-3::luc::gfp::unc-54 3'UTR, unc-119(+)] IV) were cultured in liquid culture (S-Basal supplemented with OP50, OD600=2.7, 1000 animals/mL) in a temperature controlled incubator at 25°C, shaking at 180 rpm. Every hour samples were collected from 0 h to 23h, RNA was extracted and treated with DNAse, then subjected to sequencing reactions.