Tripidium ravennae Transcriptome assembly

The Poaceae subtribe Saccharinae is diverse spanning numerous genera including Erianthus, Miscanthus, Saccharum, and Tripidium. Some tripidium species have demonstrated particular value as emerging biomass and bioenergy crops in various production systems. New advanced hybrids between T. arundinaceum and T. ravennae are under evaluation in Western North Carolina and are demonstrating high yields and overwintering survival in USDA Zone 6b. These crops may serve as an alternative to other temperate adapted perennial grass species in dedicated biomass and bioenergy feedstock systems. Having foundational genomic and translational sequence information for these novel grasses could enable innovative approaches and modern breeding applications for tripidium and related genera. However, genomic sequence assembly of heterozygous and/or polyploid species remains a challenge. T. ravennae is a diploid species (2n = 2x = 20; 1Cx = 1.015pg approx. 1Gb) of tripidium, that shares a common genetic base of ten chromosomes with Saccharum and Sorghum. The availability of a reference genome and accompanying floral development transcriptome for T. ravennae provides an opportunity for variant calling, sequencing read alignment, comparative genomics, functional analyses, and genome editing applications.

禾本科亚族Saccharinae种类繁多，涵盖了包括Erianthus、Miscanthus、Saccharum和Tripidium在内的多个属。部分Tripidium物种在多种生产系统中已显示出作为新兴生物质和生物能源作物的特殊价值。在北卡罗来纳州西部，T. arundinaceum与T. ravennae之间的新高级杂交种正在评估中，并在USDA 6b种植区展现出高产和越冬存活能力。这些作物可能成为其他温带适应的多年生草本植物在专用的生物质和生物能源原料系统中的替代品。为这些新颖的草类植物提供基础基因组学和转化序列信息，将有助于推动对Tripidium及其相关属的创新方法研究和现代育种应用。然而，杂合子及/或多倍体物种的基因组序列组装仍然是一项挑战。T. ravennae是一种二倍体物种（2n = 2x = 20；1Cx = 1.015pg，大约1Gb），与Saccharum和Sorghum共享十个染色体的共同遗传基础。T. ravennae参考基因组和伴随的花发育转录组的可用性，为变异调用、测序读段对齐、比较基因组学、功能分析和基因组编辑应用提供了机遇。