Bacterial community on brown film formed by Lentinula edodes

The pigment composition and the transcriptome associated with brown film formation have been widely studied. However, the microbiome associated with brown film in Lentinula edodes has not been examined. In this study, we performed 16S amplicon sequencing to understand the dynamics of the bacterial microbiome and select potential microbial biomarkers. on the brown film of L. edodes. To achieve this, we investigated: (1) the bacterial community, diversity, and functionality in the first and third flush of mushroom cultivation; and (2) the unique taxa in different flush by the linear discriminant analysis effect size (LEfSe) method. This study provides a foundation for the further analysis of bacterial communities of brown film of L. edodes.