Supplemental Image Data for CD23-0798 Part 1/2 "Pre-existing skin-resident CD8 and gd T cell circuits mediate immune response in Merkel cell carcinoma and predict immunotherapy efficacy"

Supplemental Image Files (slide scans and snapshots for scRNAseq, mIF, Nanostring) CD23-0798 “Pre-existing skin-resident CD8 and gd T cell circuits mediate immune response in Merkel cell carcinoma and predict immunotherapy efficacy.”

The files are distributed across multiple directories with the listed subdirectories:

This is dataset 1/2.

mIFSnapShots – these are representation snapshots of multiplex IF images used for analysis

a. Subdirectory: Trm (patients with evaluable response)

i. 29 snapshots (.jpg, 2 per sample for all except one) = 57 files representing 29 samples from 26 patients

1. 1 set (MO_068) of skin and LN

2. 2 sets of pre-post (MO_079 and MO_085)

b. Subdirectory: GammaDelta

i. 8 snapshots ( .jpg, 2 per sample) = 16 files representing 8 samples from 7 patients

1. 1 set of pre-post pair (MO_085)

NanostringSpatial (GeoMx) – these files show annotation of ROI used for analysis in the context of tissue.

GeoMx

i. File: marker_color_key.xlsx

ii. Subdirectory: CTA (14 files, 9 patients)

iii. Subdirectory: WTA (9 files, 3 patients)

1. 2 pre-post pairs (MO_46, MO_51)

scRNAseqHEscans – 44 total scan files (.svs) – divided across 4 compressed (.zip) directories

a. Northwestern: 2 whole-slide H&E scans (.svs) = 2 files representing 2 samples from one patient (NU_44) – Post1 and Post2

b. Moffitt: 42 whole-slide H&E scans (.svs) (1 per sample) = 42 files representing 42 samples from 38 patients

i. 2 sets of skin / LN (MO_068 and MO_082)

ii. 2 sets of pre-post (MO_079 and MO_085)