Bacterial community in the plastisphere and water with different urbanization levels

Approximately 30 grams of polyethylene (PE) or polystyrene (PS) MPs with ≦ 75 μm diameter were sealed in a plastic bag with ≦ 30 μm apertures. In this study, Shidou reservoir (N 24o 42’ 35’’, E 118o 0’ 14’’,) and Xinglin bay (N 24o 35’44’’, E 118o 5’ 34’’) were selected due to their presence in the same watershed with different urbanization in Xiamen, Fujian province of China. Each microplastic with triplicates was immersed in the water of Shidou reservoir and Xinglin bay, respectively. After 30 days, the MPs and their corresponding water were collected and used for microbial genomic DNA extraction in November 2020.We amplified bacterial 16S rRNA genes using the primer set of 338F/806R to characterize the community of bacteria in water and plastisphere.