DEtail-seq profiled meiotic DSBs in Mus musculus

SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for dissecting the molecular mechanisms behind. Here, we report a novel technique, named DEtail-seq (DNA End tailing followed by sequencing), that can directly and quantitatively capture the meiotic DSB 3' overhang hotspots at single-nucleotide resolution. Overall design: Profiled mammalian meiotic DNA breaks by performing DEtail-seq in mouse sertoli cells (SC), testicular cells at leptotene/zygotene stages (L/Z), and pachytene stage (Pac)