T-UCR and related gene expression quantification in the presence or not of CpG-ODN oligonucleotide 2006

Toll-like receptors (TLRs) are mediators in the innate immune response by recognizing nucleic acids derived from microbial components as for TLR9 binding ability for CpG rich bacterial DNA. We studied the differential expression by microarrays of all known T-UCRs and near genes in a series of 6 primary B-CLL cases cells comparing cells treated in vitro with CpG ODN versus mock controls. CpG oligodeoxynucleotide (ODN) is a synthetic TLR9 agonist that has been considered for the treatment of several cancer types, including relapsed or refractory B-cell chronic lymphocytic leukemia (B-CLL) by their impact in proliferation and viability. Nevertheless, boosting of CpG ODN response for an effective therapeutic response depends on increasing the knowledge of the molecular pathways affected by this compound.