Transcriptome analysis of nascent hematopoietic stem cells (HSCs) induced by the ectopic expression of Evi1. Transcriptome analysis of nascent hematopoietic stem cells (HSCs) induced by the ectopic expression of Evi1

To examine the role of Evi1 in HSC specification, we generated Tie2-Cre::ROSA-Evi1 mice, which can induce Evi1 in endothelial cells. We observed five-fold increase of HSCs In Tie2-Cre::ROSA-Evi1 embryo. To characterize the induced HSCs in Tie2-Cre::ROSA-Evi1 embryos, we analyzed the gene expression profile of HSCs. Overall design: HSCs were isolated from Tie2-Cre::ROSA-Evi1 and ROSA-Evi1 fetal livers, and were subjected to RNA-seq analysis.