Distinct Functional Properties of Perinatal vs. Adult Adipose Progenitors

We previously identified functional distinct sub-populations in adult male gWAT Pdgfrb+ cells utilizing the MuralChaser lineage tracking system and single-cell RNA-seq. Later in this related submission, we performed single-cell RNA-seqs on different developing stage of gWAT (P3, P7 and 5-week). We identified 4 sub-populations of Pdgfrb+ cells. Bulk-mRNA were conducted to these sub-populations in order to understand their transcriptomic profiles. Overall design: Bulk mRNA-seq was done on freshly isolated Pdgfrb+ sub-populations (adipocyte precursor cells, fibro-inflammatory progenitors, mesothelial-like cells, smooth muscle cells) by FACS.