Macrogenomics-based analysis of the effects of intercropped soybean photosynthetic characteristics and nitrogen-assimilating enzyme activities on yield at different nitrogen levels

We collected inter-root soils of soybean at the flowering stage based on six consecutive years of maize/soybean intercropping planting pattern, and investigated the effects of intercropping and nitrogen application rate on photosynthetic characteristics, soil nitrogen content, and nitrogen assimilating enzyme activities, nitrogen-assimilating enzyme activities and microbial community composition of soybean, which were correlated with yield.