Transcription in wounded rat vocal folds and vocal fold fibroblasts

We used microarrays to characterize transcriptome profiles of rat vocal fold tissue following surgical injury (vs. naive tissue); rat vocal fold fibroblasts harvested from scar tissue at the 60 d time point (vs. naive fibroblasts); rat vocal fold scar fibroblasts treated with siRNA against the collagen chaperone protein rat gp46 (vs. scramble siRNA).本研究采用基因芯片（microarray）技术表征了三类大鼠样本的转录组图谱：分别为手术损伤后的大鼠声带组织（以未处理的正常声带组织作为对照）、术后60天时间点从瘢痕组织中分离获取的大鼠声带成纤维细胞（以未处理的正常成纤维细胞作为对照），以及经靶向胶原伴侣蛋白大鼠gp46的小干扰RNA（small interfering RNA，siRNA）处理的大鼠声带瘢痕成纤维细胞（以乱序小干扰RNA（scramble siRNA）处理的细胞作为对照）