Genome analysis of Thermosulfuriphilus ammonigenes ST65T, an anaerobic thermophilic chemolithoautotrophic bacterium isolated from a deep-sea hydrothermal vent

Thermosulfuriphilus ammonigenes ST65T is an anaerobic thermophilic bacterium isolated from a hydrothermal vent chimney located at the Eastern Lau Spreading Centre in the Pacific Ocean, at a depth of 1,870 m. Here, we present the complete genome sequence of strain ST65T. The genome consists of a single chromosome of 2,287,345 base pairs in size and has a G+C content of 51.9 mol%. The genome encodes 2,172 proteins, 48 tRNA genes, and 3 rRNA genes. Genome analysis revealed a complete set of genes essential to CO2 fixation and gluconeogenesis. Homologs of genes encoding known enzyme systems for reduced sulfur compounds oxidation and nitrate ammonification are absent in the genome of T. ammonigenes assuming unique mechanisms for these pathways. This is the first reported genome of a bacterium from the genus Thermosulfuriphilus, providing insights into the microbial contribution into carbon, sulfur and nitrogen cycles in the deep-sea hydrothermal vent environment.