Differential gene regulation and tumor inhibitory activities of alpha-, delta- and gamma-tocopherols in estrogen-mediated mammary carcinogenesis

Purpose: Examination of different forms of tocopherol treatment in mammary tumorigenesis by comparing NGS-derived transcriptome profiling (RNA-seq) Methods: Use of cDNA library construction and Illumina sequencing (NextGen 75 bp pair-end) and quality control using FastQC. Results: Differential transcriptome profiling of mammary tumors between different forms of tocopherol treatment was observed. Conclusions: Our study represents the first detailed analysis of mammary tumor transcriptomes with different tocopherol treatment generated by RNA-seq technology. The optimized data analysis reported here should provide a framework for comparative investigations of expression profiles with tocopherols. Overall design: mRNA profiles of mammary tumors from ACI rats treated with different tocopherols for 30 weeks were generated by cDNA library construction/illumina sequencing