Dataset on the content of vitamin D3 and 25-hydroxyvitamin D3 in 40 pork (Breitov breed) commodities

<i>This dataset demonstrates the content of vitamin D</i><i>₃</i><i> and 25-hydroxyvitamin D</i><i>₃</i><i> (25OHD</i><i>₃</i><i>) in boiled, grilled, and raw pork for both lean and whole cuts</i><i> including ribs, tenderloin, shoulder, neck, belly, and center chops</i><i>. </i><i>Total fat content in raw pork cuts is determined by the gravimetric method.</i><i></i> <i>Quantification analysis using reverse phase </i><i>high performance</i><i> liquid chromatography with UV-DAD</i><i> connected</i><i>.</i><i> </i><i>Purification of fatty acids from proteins was performed using a c18 cartridge, resolving vitamin D</i><i>₃</i><i> from its metabolites by subjecting purified samples to polar silica cartridge, purification from sugars by a series of two amino columns. Quantification of </i><i>vitamin D₃ and </i><i>25-hydroxyvitamin D</i><i>₃</i><i> by injecting purified extracts on C18 SPE column.</i><i> </i><i>Grilling samples was conducted in an electric combi oven.</i><i></i>

本数据集涵盖了水煮、烤制及生鲜猪肉的瘦切块与整切块中维生素D3（vitamin D₃）和25-羟基维生素D3（25-hydroxyvitamin D₃，25OHD₃）的含量数据，所涉猪肉部位包括肋排、里脊、肩肉、颈肉、腹肉与中心猪排。生鲜猪肉切块的总脂肪含量采用重量法进行测定。采用搭载紫外二极管阵列检测器（UV-DAD）的反相高效液相色谱（reverse phase high performance liquid chromatography）开展定量分析：通过C18固相萃取柱（C18 cartridge）完成蛋白质中脂肪酸的纯化；将纯化后的样品通过极性硅胶柱（polar silica cartridge）实现维生素D3与其代谢产物的分离；再通过两支串联的氨基柱（amino columns）完成糖类杂质的去除。将纯化后的提取物注入C18固相萃取柱（SPE column），即可完成维生素D3与25-羟基维生素D3的定量分析。烤制样品的制备在电热组合烤箱（electric combi oven）中完成。