Pandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled

The aim of this project is to determine the protein changes of P. amaryllifolius in response to drought stress and during recovery. The protein changes between drought-stressed, well-watered, and recovered plants were evaluated using tandem mass tags (TMT)-based quantitative proteomics. The proteins were extracted from sample by using TCA/Acetone method and digested with trypsin. The digested proteins were then labelled with 10-plex TMT labelling prior fractionation with 12.5%, 17.5%, and 50% ACN with 0.1% triethylamine. The prepared proteins were then subjected to 1D data dependent acquisition (DDA) with nano LC column ionization source was set as positive ion mode, whereby 350-1850 m/z peptide precursors were scanned at 70,000 resolutions. The raw data generated was then searched against Viridiplantae protein sequences downloaded from UniProt. Of the 1,415 differentially abundant proteins, 74 were significantly altered. The majority of proteins differing between them were related to carbon metabolism, photosynthesis, stress response, and antioxidant activity. This is the first study that reports the protein changes in response to drought stress in Pandanus. The data generated provide an insight into the drought-responsive mechanisms in P. amaryllifolius.