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Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

ChEBI2013-08-10 更新2026-07-21 收录

CRISPR转录激活

内源基因调控

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-GEOD-49701 数据链接链接失效反馈

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资源简介：

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

应用场景：

创建时间：

2013-08-10

脆性X综合征基因治疗

CRISPR转录激活

The aim of this work is to selectively increase transcription from the FMR1 locus in FXS hESC lines using CRISPR-Cas9 fused to transcriptional activators. Endonuclease deficient Cas9 (dCas9) was fused

NIAID Data Ecosystem50

Comparative Analysis of Cas9 Activators Across Multiple Species

CRISPR转录激活

基因表达调控

RNA-Seq after Cas9-gRNA transfection with different activators targeting HBG1 we performed PolyA Selection and RNA-Seq on cells transfected with a dCas9-based transcriptional activator (VP64, VPR, SAM

NIAID Data Ecosystem40

CRISPR-assisted transcription activation by phase separation proteins

CRISPR转录激活

基因编辑脱靶检测

The cells co-expressing dCas9-VPR or dCas9-VPRF with the OCT4-targeting gRNA were harvested for RNA-seq analysis to detect the off-target activation of the systems.

NIAID Data Ecosystem30

Programmable CRISPR-Cas transcriptional activation in bacteria. Escherichia coli BW25113

合成生物学

CRISPR转录激活

Engineering synthetic CRISPR activators for bacteria

NIAID Data Ecosystem40

Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions

基因调控机制

CRISPR转录激活

Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of th

NIAID Data Ecosystem30

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

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