Single-cell RNA-Sequencing of Wild-type Perinatal Mice Thymi
收藏Alliance of Genome Resources2026-08-01 收录
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We prepared enzymatically digested thymus single cell suspensions for scRNA-Seq. Cells were extracted from perinatal mice, and TEC cells were enriched via FACS using anti-EpCAM and Anti-CD45 to isolate EpCAM+ CD45- TECs. FACS purified TECs were obtained from P0, P3, P7, P10, P15 and P28 C57Bl6/J male and female thymuses. Supplicate experiments were performed for each time point except for P15.
