A study of transcriptome response to salt treatment across three zones of the root in a barley landrace and malting cultivar. A study of transcriptome response to salt treatment across three zones of the root in a barley landrace and malting cultivar

Paired-end RNA-Seq libraries were constructed for three root sections of the roots of barley cv. Clipper, and landrace Sahara, grown under control and salt-treated (100 mM NaCl) conditions on agar plates in quadruplicate. Experiments were conducted in a temperature-controlled growth cabinet at 17 °C in the dark. After three days of germination, seminal roots were dissected according to the following steps: A 1.5 mm long section marked ‘Zone 1’ (meristematic zone) was taken from the root tip. A second section (‘Zone 2’) was dissected from the elongation zone up to a third section, ‘Zone 3’ (maturation zone), which was excised at the point of visible root hair elongation up to 3⁄4 of the entire root. Four biological replicates were generated for each sample in four separate experiments totaling 48 samples. All RNA-seq libraries were constructed and paired-end sequenced (100 bp) on an Illumina HiSeq 2000 system at the Australian Genome Research Facility (Melbourne, Australia).