A mannose-sensing AraC-type transcriptional activator regulates intestinal colonization of Vibrio cholerae

In this study, we show that ManR (VC1825) activates the transcription of the mannose operon in M9 medium supplemented with fructose or mannose, and when manR is impaired Vibrio cholerae cells fail to colonize the intestine and host survival is significantly prolonged in a fruit fly Drosophila melanogaster infection model. To identify other target genes of ManR, transcriptome analysis was performed in wild-type V. cholerae and manR mutant strains, , grown on M9 medium supplemented with glucose, fructose, or mannose. Transcriptome profiles of WT V. cholerae and manR mutant cells grown on a single carbohydrate as a sole carbon source (glucose, fructose, mannose)