Expression data from BY4743 and dbr1 yeast

Introns in pre-mRNAs must be spliced out prior to their translation. During splicing, introns are removed in the form of a lariat, in which the 5' end is linked to the 2' hydroxyl of an internal adenosine. Lariat degradation is initiated by an 2'-5' phosphodiester-specific RNA endonuclease which debranches these lariat RNAs to linear form. Deletion of the debranching enzyme is yeast results in the accumulation of lariat introns. We used this accumulation to identify spliced lariat introns on a genome-wide scale in S. cerevisiae using tiling microarrays. Keywords: two sample comparison, 3 biological replicates Total RNA from BY4743 and dbr1 yeast was harvested, labeled as double-stranded probes and hybridized to Affymetrix tiling microarrays. Signals enriched in the dbr1 strain were mapped to genomic coordinates to assess correspondence with known intron regions and identify novel spliced sequences.