melanoma and melanocytes sequencing

We performed RNA deep sequencing on poly-A RNAs from three normal melanocyte primary cultures and three melanoma cell lines. The three melanoma cell-lines were recently established from lymph node metastases of melanoma patients. In addition, in terms of mutations of the raf pathway, one cell-line was not mutated (M383), one had Braf V600E mutation (M305) and the last one harbored N-ras Q61R mutation (M315). Our primary objective was to identify cancer specific neoantigens whose expression is controlled by an IRES-dependent translation. Initial selection of transcripts of interest was performed based on RNA-seq results presented here.