RNA-seq for cold tolerance in B. napus

Winter rapeseed is an important winter over crop, whose distributions and productions are affected by low temperature. To gain insights into the transcriptomic changes in B. napus with varying cold tolerance, comparative transcriptome analysis in leaves was performed between GZ hui and 10B which exhibit a clear difference in cold tolerance. GZ hui and 10B, at a six-leaf stage, were treated under cold treatments in the artificial climate chamber, 20 \u00b0C (as CK), 5 \u00b0C for 2h (as T1), T1 + 0 \u00b0C 2h (as T2), and T2 + -5 \u00b0C 2h (as T3) with three replicates. These studies lay a good foundation for cloning and understanding of the molecular mechanism of cold tolerance genes in B. napus.