Leishmania guyanensis CL085 genome assembled from short read data provided by the Sanger Institute. Leishmania guyanensis CL085 genome sequencing

Leishmania DNA was sampled from two dogs with the cutaneous form of canine leishmaniasis in Colombia in 1985/86 and whole genome sequenced. We assembled the genomes to chromosome level with short-read data, using both de-novo assembly and reference based alignment, and annotated them, finding over 8,000 genes on each genome. A control assembly created from short-reads, using the same pipeline, was also used to validate results. The genomes were identified as L. guyanensis and L. naiffi, using a phylogenomics approach. These are both members of the Viannia subgenus of Leishmania which is only found in the Americas, mainly South America.