Genetic polymorphism of 17 microsatellites screened with 18 reference blood DNA extracts of black bears.

1—observed number of alleles
2—effective number of alleles
3—observed heterozygosity
4—expected heterozygosity
5—polymorphic information content
6—inbreeding coefficient
7—probability of identity (locus)
8—probability of identity for sibs (locus)
9—probability of identity (cumulative)
10—probability of identity for sibs (cumulative)
11—allelic dropout rate
12—false allele rate
13—predicted frequencies of null alleles.
H—locus used for individual identification
† HWE deviation (P <0.05).
* Significance level was calculated using 10, 000 randomization, 500 batches and 10,000 iterations (P < 0.01).
Annealing temperature (TA) for multiplexes–MP 1 (50°C), MP 2 (56°C), MP 3 (64°C), MP 4 (45°C), MP 5 (62°C) and MP 6 (55°C).