Role of Retinol Saturase (RetSat) in brown adipose tissue (BAT).

This study investigates the transcriptional consequences of genetic RetSat deletion in BAT. Male C57BL/6J mice with floxed RetSat alleles with (Cre+) or without (Cre-) Ucp1-Cre were analyzed at an age of 12-14 weeks. Total RNA from BAT was isolated and RNA was DNAse digested and profiled by RNAseq.本研究探究了棕色脂肪组织（brown adipose tissue, BAT）中RetSat基因遗传性敲除所产生的转录组学效应。实验选取12~14周龄的雄性C57BL/6J小鼠，分为两组：携带floxed RetSat等位基因并带有Ucp1-Cre的Cre+组，以及携带floxed RetSat等位基因但不带有Ucp1-Cre的Cre-组，随后对两组小鼠开展相关分析。提取两组小鼠BAT的总RNA，经脱氧核糖核酸酶（DNase）消化后，通过RNA测序（RNA-seq）进行转录组分析。