Dissecting cell composition and cell-cell interaction network of normal human heart tissue by single-cell sequencing

We studied the cell compositon of normal human heart by single-cell sequencing. Distint subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We drawed a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with age. Overall design: Extract cells from left ateria and ventricle of normal heart and conduct single-cell sequencing of 9994 cells.