HIF-1 regulates pathogenic T cells in lupus skin disease (scRNA-Seq)

Using a mouse model of lupus skin disease, we find that skin-infiltrating CD4+ and CD8+ T cells express high levels of HIF-1, demonstrate a strong cytotoxic signature, and the cytotoxic signature decreases after pharmacologic HIF1 inhibition. Overall design: We performed scRNA seq of MRL/lpr splenocytes and skin cells to understand the role of HIF1 in promoting lupus skin disease. We combined this technology to CITE-sequencing to obtain measurement at the protein and transcriptome level.