Effect of γ-secretase inhibition on Hath1/MUC2/P27Kip1 expression in human colonic carcinomas in primary culture.

*NOS: not otherwise specified (WHO classification), u = unresponsive tumor culture.A: Characteristics of the primary tumors: histopathological diagnosis was according to the WHO classification of gastrointestinal tumors [42] and immunohistochemistry of nuclear β-catenin. B: Determination of Hath1, MUC2, P27Kip1 mRNA levels in control 24 h cultures was performed by quantitative RT-PCR. Results are normalized to β-actin gene expression. Arbitrary units were translated into a 3-tier scale; (−): absence of expression, (+): moderately expressed (1–10 arbitrary units), (++): highly to very highly expressed (20–100 arbitrary units). C: RT-PCR detection of Hes1, Hath1, MUC2 and P27Kip1 in 10 human colon carcinomas in primary culture with or without DBZ for 24 hours. Response to DBZ was expressed as the fold change versus control (Mean of 3 determinations).