Proteomic analysis of mouse astrocytes and their secretome by a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.

Data from ProteomeXchange, PXD ID: PXD000501. C8-D1A cells analyzed. Experiment: C8D1A_secretome_set1_2, file: 20130530_C8D1A_secretome_set1_F6_2.mzml. Published as part of Proteomics. 2014 Apr 17 . From the Abstract: {{i}} Astrocytes are the most abundant cells in the CNS, but their function remains largely unknown. Characterization of the whole-cell proteome and secretome in astrocytes would facilitate the study of their functions in various neurodegenerative diseases and astrocyte-neuron communication. To build a reference proteome, we established a C8-D1A astrocyte proteome to a depth of 7265 unique protein groups using a novel strategy that combined two-step digestion, filter-aided sample preparation, StageTip-based high pH fractionation, and high-resolution MS. {{/i}}