Photoperiod-mediated Chromatin Accessibility in the Single Cell Types of Leaf Phloem and Epidermis in Arabidopsis

Photoperiodic control of the phase transition from vegetative to reproductive growth plays a key role in high plants. Although leaves are considered to be the major organs to perceive the day-length signal, it is not clear what the specific responses happen in the vasculature and epidermis tissues. Here, we performed ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) and RNA-seq with purified phloem and epidermis nuclei by applying INTACT (isolation of nuclei tagged in specific cell/tissue types) sorting method. We found that accessible chromatin regions (ACR) in phloem showed higher variations than epidermis during sensing the long-day signal. These cell/condition-type-specific ACR changes are basically related with gene expression. Taken together, our cell type specific studies sheds light on photoperiodic control of the flowering transition and also investigating of plant development mechanism. Overall design: ATAC-seq and RNA-seq in 2 cell types.