Additional file 1 of A molecular epidemiological study on Escherichia coli in young chicks with colibacillosis identified two possible outbreaks across farms

Additional file 1. Metadata linked to each isolate sequenced in this study including flock and sampling data, bacteriological grading, sequencing quality measures, virulence-associated genes identified and accession numbers to ENA. 1The sample ID explains from what flock (first number) and bird (last number) the isolate was sampled. 2The bacterial culture was graded according to purity of growth on three agar plates incubated over night in either normal oxygen pressure, CO2 chamber or anaerobically: grade 1 = pure growth, grade 2 = few colonies of Enterococcus spp. or Proteus spp. were present on the agar, together with an otherwise pure culture of E. coli. Grade 3 = dominating growth of E. coli with sparse to medium growth of either Enterococci spp, Proteus spp. or less growth of a different bacteria, grade 4 = mixed culture: minimum of three different bacteria, where E. coli was not the dominating bacteria. 3The presence of pathological lesions associated with colibacillosis (colisepticaemia) within an individual bird during necropsy, 1 if ≥2 lesions present, 0 if <2 lesions present. 4Each individual bird received a colibacillosis diagnosis (1) if the bird had lesions of colisepticaemia and the bacteriological examination from the individual sample was graded 1-3.