Whole transcriptome RNA sequencing of E16.5 Six2TGC and Six2TGC;miR-17~92fl/fl nephron progenitors

The goal of this study is to identify dysregulated mRNAs and lncRNAs in nephron progenitors as a consequence of a loss of miR-17~92 post-transcriptional regulation Nephron progenitors were FACS sorted from E16.5 Six2TGC (Control) and Six2TGC;miR-17~92fl/fl (Mutant) kidneys based on the GFP driven by the Six2 locus. A total of 3 replicates per genotype were used for the whole transcriptome RNA sequencing. Each replicate consists of nephron progenitors pooled from 4 embryos, resulting in 12 samples per genotype assayed in this manner.本研究旨在鉴定肾单位祖细胞中因miR-17~92转录后调控缺失所导致的失调信使RNA（mRNA）与长链非编码RNA（lncRNA）。本研究基于Six2基因座驱动的绿色荧光蛋白（GFP）标记，从胚胎期16.5天（E16.5）的Six2TGC（对照组）及Six2TGC;miR-17~92fl/fl（突变组）肾脏中，通过荧光激活细胞分选（FACS）分离肾单位祖细胞。每组基因型设置3次生物学重复，用于开展全转录组RNA测序。每份重复样本由4个胚胎的肾单位祖细胞混合制备，最终每组基因型共获得12份待测样本。