Functional disruption of a putative pseudouridine synthase impairs Pi starvation responses in Arabidopsis.

To investigate the molecular mechanism by which the DPS1 mutation affects plant responses to Pi starvation, we performed a comparative RNA sequencing (RNAseq) analysis of the root transcriptomes. Complementary DNA libraries were constructed using the total RNAs isolated from the root of 7-d-old seedlings growing on P+ or P– media.