Transcriptional response to MMS in haploid and diploid Schizosaccharomyces pombe

Polyploidy has been implicated in genome instability and tumorigenesis. We use Schizosaccharomyces pombe diploids as a model for studying the consequences of whole genome duplications on genome integrity. In this study, our aim is to investigate the transcriptional profile between haploid and diploid S. pombe in unperturbed and MMS treated conditions (0.0075% MMS, 4 hours @ 32 degrees Celsius in YES media). Investigate the transcriptional profile between haploid and diploid S. pombe in unperturbed and MMS treated conditions (0.0075% MMS, 4 hours @ 32 degrees Celsius in YES media). Total RNA was extracted using Qiagen RNeasy kit. ERCC spike-ins were added in equal volume to all samples. Samples were sent to Novogene for mRNA purification (polyA selection), cDNA synthesis, library preparation, and paired-end sequencing.