Summary of peptide characteristics.

Peptides containing HLA-E binding motifs were predicted in silico using motif scores, discriminant analysis and diversity analysis. Peptide sequences are indicated as well as the proteins their originating proteins (Mtb Rv numbers and their Swissprot accession numbers). Binding was tested by HPLC size exclusion chromatography using recombinant human HLA-E and competition of a fluorescently labeled peptide [33]. Binding was calculated as the concentration (µM) of peptide required to reduce fluorescence intensity of the standard peptide with 50% (IC50).
*UniProtKB/ Swissprot accession number.
aIdentified through “motif” score, i.e. weighting positions differently.
bTop five from a discriminant analysis: binder vs non-binder.
cTop Twenty-odd from diversity analysis of peptide sequences with high discriminant scores.
NA, not available.