Effect of rpoS inactivation on reporter fluorescence from a single-copy Pint-egfp; PinR-eCherry fusion in P. knackmussii strain B13 and derivatives.

1)Time after culture inoculation. Time 24 h is onset of stationary phase.2)Average eGFP or eCherry (in bold) fluorescence (relative units) within the subpopulation of cells across biological triplicates (see Figure S7 for explanation).3)Average subpopulation of cells (percent of total) expressing egfp from Pint (or eCherry from PinR, in bold) determined from cumulative distribution curves among biological triplicates.4)Significance of difference (P5)Calculated P-values in pair-wise homoscedastic T-test between proportions of eGFP and eCherry expressing cells.