RNA-seq of Serine metabolism reprogramming in colorectal cancer

We performed transcriptome profiling by RNA sequencing (RNA-seq) in SW620-S and SW620 cells, to gain insight into the molecular basis of serine deprivation resistance. To examine the hypothesis that increased PHGDH meets the requirement of cells cultured in serine-depleted media for multiplication, we treated cells with CBR5884 and performed transcriptome profiling. YAP was activated in SW620-S cells. To study the role of YAP in serine deprivation resistant cells , we treated cells with Verteporfin(VP) to explore the changes of genes.