Transcriptome of soybean reproductive tissues from plants subjected to water deficit, heat stress and a combination of water deficit and heat stress.

Soybean plants were subjected to water deficit, heat stress, and combination of water deficit and heat stress. Flower parts, sepal, anther, ovary and stigma were collected from 8-10 different plants at R1 stage growing under three above mentioned stress conditions, and under control conditions 10 days after initiation of the stresses. Differential gene expression compared to control was studied using RNAseq method. Overall design: Soybean plants (16-18 days old) were subjected to water deficit, heat stress, and combination of water deficit and heat stress and flower parts were collected in liquid nitrogen. Total RNA was isolated and mRNA profile were generated by deep sequencing using NovaSeq 6000 PE150.