Gene expression profiling of E1A;HrasG12V mouse embryonic fibroblasts grown under physiological (5%) oxygen conditions, with wild-type p53 or p53 null

To investigate how pleiotropic the impact of p53 loss is on cellular function, generated isogenic, polyclonal wild-type (sgNTC) and p53-null (sgp53) E1A;HrasG12V mouse embryonic fibroblast cell lines using CRISPR/Cas9. Here we show gene expression analysis on p53 wild-type (sgNTC) and p53null (sgp53) E1A;HrasG12V MEFs grown under physiological oxygen conditions (5% oxygen). Examination of gene expression in p53 wild-type and p53-null E1A;HrasG12V MEFs, grown in physiological oxygen (5% oxygen)