Expression data from HEK-293 cells with and without small hepatitis delta antigen (S-HDAg) overexpression

Hepatitis delta virus (HDV) enters hepatocytes via the receptor NTCP and requires the SHDAg for efficient replication by host RNA polymerase II (Pol II). During replication, SHDAg reportedly binds to Pol II as an elongation factor and thus promotes the transition from the promoter-proximal pausing to the elongation state. Here, we are interested in looking at the potential of S-HDAg as a global regulator of Pol II-mediated transcription in the presence and absence of the Mediator kinases CDK8/19 SHDAg or an empty vector was stably transduced in HEK-293-NTCP wild-type and CDK8/19 dKO cell lines by lentivirus. Stable cell lines were subsequently infected with 10 MGE of HDV or with DMEM medium only. At 5 days post-infection, cells were harvested for RNA extraction and subjected to the human Clariom S assay, ThermoFisher.