Sporulation efficiency and spore viability in SUMO-diminished strains.

Viability of spores produced by diploid SMT3 homozygotes, smt3Δ/+ heterozygotes, PSCC1[SMT3]/PSCC1[SMT3] homozygotes, or PSCC1[SMT3]/smt3Δ transheterozygotes. The far right column shows the overall spore viability of each strain. Note that as smt3 haploid cells are inviable, 50% overall spore viability is the maximum expected for strains carrying an smt3 deletion allele. Displayed in each “Distribution of tetrad types” column is the frequency of tetrads containing four viable spores (4-sv), three viable spores (3-sv), two viable spores (2-sv), one viable spore (1-sv) or no viable spores (0-sv). Full strain genotypes are listed in Table S1.
#Sporulation efficiency in this case was performed on K199, which has the same genotype as K325 but was generated independently.
*Cases of 3- or 4-spore viables derived from strains carrying smt3::natMX (K196, K197, K224) arose from non-mendelian segregation events; each colony from dissected ascii displayed sensitivity to nourseothricin, indicating an absence of the smt3::natMX knockout allele.