Mineralocorticoid receptor co-expression effect on ligand-induced glucocorticoid receptor interaction with the genome in human cell line

Our previous results observed glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) physical and functional interactions. RNAseq study (GSE163506) revealed that MR blunted GR ligand-dependent gene regulation in human cell lines. In the current study, we developed U-2 OS cell line-derived cells constitutively expressing GR or MR plus GR. Then, we compared GR – genome interaction before and after dexamethasone treatment. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for glucocorticoid receptor in GR clell and MR/GR cell