Single cell transcriptomic analysis of human axioloids

We have established a pluripotent stem cell (PSC)-based 3D model of human axial development, which recapitulates in vitro key features of the somitogenesis process including axial elongation, segmentation and sequential formation of epithelial somites with proper rostrocaudal pattering, and traveling wave-like gene expression dynamics associated with the segmentation clock. Initial molecular and functional analysis of these axially-organized embryonic tail-like structures, which we termed axioloids, revealed the spatiotemporally controlled self-organization of somitogenesis associated human mesodermal cell populations. In order to gain a deeper understanding this model, we performed temporally resolved (10X-based) single cell RNA-seq (scRNA-seq) and analyzed axioloids at various timepoints (48h, 72h, 96h & 120h) and conditions (-MG vs. +MG alone or MG + Retinoic Acid (RA) or Retinal (RAL)). Overall design: Time-resolved (10X) single cell transcriptomic analysis of human axioloids